Signalling

Part:BBa_K4998019:Design

Designed by: Rafail Andreou   Group: iGEM23_thessaloniki   (2023-10-09)


LipA-Tat Pathway


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A signal peptide's proper function depends on the protein it is fused with. After bibliographic research, we found out that the lipA peptide sequence seemed to be successful for a wide variety of proteins in Bacillus subtilis.


Source

Bacillus subtilis

References

[1] Monteferrante, C. G., MacKichan, C., Marchadier, E., Prejean, M.-V., Carballido-López, R., & van Dijl, J. M. (2013). Mapping the twin-arginine protein translocation network of Bacillus subtilis. PROTEOMICS, 13(5), 800–811. https://doi.org/10.1002/pmic.201200416

[2] Zhang, W., Yang, M., Yang, Y., Zhan, J., Zhou, Y., & Zhao, X. (2016). Optimal secretion of alkali-tolerant xylanase in bacillus subtilis by signal peptide screening. Applied Microbiology and Biotechnology, 100(20), 8745–8756. https://doi.org/10.1007/s00253-016-7615-4